Microbiology

Microbiology in General

Electrolytically generated Ag+ and Cu+ ions are potently microbiocidal although somewhat slower acting than chlorine they are much less subject to degradation, having residual antimicrobial potency lasting for months. The recommended minimum level of free chlorine for disinfection of public swimming pools is 1 mg/liter (1 ppm). This level is difficult to maintain due to the chlorine-demanding organic material introduced by bathers themselves as well as the environment. Consequently the residual level of chlorine will have often dropped to 0.1 to 0.3 mg/liter after 24 hours.

Eye and skin irritation may also occur at the minimum chlorine level needed for effective disinfection.  Under Staphylococcus challenge, the combination of Ag+ and Cu+ together with free chlorine had a faster log 10 reduction of microbial numbers than did treatment with a high level of chlorine alone. This combination allows for a much reduced or zero level of chlorine.

Medical, Microbiology, Diseases and Infections

Anti-Microbial effects of trace quantities of metals such as Ag+ and Cu+ in trace amounts of less than 1 ppm have proven activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus anthracis, B. cereus, and B. subtilis, Vibrio cholerae, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, coagulase-negative staphylococci, and Enterococcus faecium, Candida albicans and C. maltosa, Legionella pneumophila, hepatitis A virus (HAV), human rotavirus (HRV), human adenovirus, and poliovirus (PV)aflatoxin production by Aspergillus parasiticus.

Schierholz JM, Beuth J, Pulverer GKönig DP.

Silver-Containing Polymers

Antimicrob Agents Chemother. 1999 Nov; 43(11): 2819-2821.
PMCID: 89571

Alt V, Bechert T, Steinrücke P, Wagener M, Seidel P, Dingeldein E, Domann E, Schnettler R.

In Vitro Testing of Antimicrobial Activity of Bone Cement.

Antimicrob Agents Chemother. 2004 Nov; 48(11): 4084-4088.
PMCID: 525460

Simonetti N, Simonetti G, Bougnol F, Scalzo M.

Electrochemical Ag+ for preservative use.

Appl Environ Microbiol. 1992 Dec; 58(12): 3834-3836.
PMCID: 183190

Butkus MA, Labare MP, Starke JA, Moon K, Talbot M.

Use of Aqueous Silver To Enhance Inactivation of Coliphage MS-2 by UV Disinfection.

Appl Environ Microbiol. 2004 May; 70(5): 2848-2853.
PMCID: 404450

Lin YS, Vidic RD, Stout JE, Yu VL.

Negative Effect of High pH on Biocidal Efficacy of Copper and Silver Ions in Controlling Legionella pneumophila.

Appl Environ Microbiol. 2002 Jun; 68(6): 2711-2715.
PMCID: 123931

MacKeen PC, Person S, Warner SC, Snipes W, Stevens SE Jr.

Silver-coated nylon fiber as an antibacterial agent

Antimicrob Agents Chemother. 1987 Jan; 31(1): 93-99.
PMCID: 174659

Li XZ, Nikaido H, Williams KE.

Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins.

J Bacteriol. 1997 Oct; 179(19): 6127-6132.
PMCID: 179518

Kaur P, Vadehra DV.

Mechanism of resistance to silver ions in Klebsiella pneumoniae.

Antimicrob Agents Chemother. 1986 Jan; 29(1): 165-167.
PMCID: 180387

Matsumura Y, Yoshikata K, Kunisaki SI, Tsuchido T.

Mode of Bactericidal Action of Silver Zeolite and Its Comparison with That of Silver Nitrate.

Appl Environ Microbiol. 2002 Jun; 68(6): 2711-2715.
PMCID: 123931

Gupta A, Maynes M, Silver S.

Effects of Halides on Plasmid-Mediated Silver Resistance in Escherichia coli.

Appl Environ Microbiol. 1998 Dec; 64(12): 5042-5045.
PMCID: 90966

Riggle PJ, Kumamoto CA.

Role of a Candida albicans P1-Type ATPase in Resistance to Copper and Silver Ion Toxicity.

J Bacteriol. 2000 Sep; 182(17): 4899-4905.
PMCID: 111370

Kampf G, Dietze B, Große-Siestrup C, Wendt C, Martiny H.

Microbicidal Activity of a New Silver-Containing Polymer, SPI-ARGENT II.

Antimicrob Agents Chemother. 1998 Sep; 42(9): 2440-2442.
PMCID: 105850

Klaus T, Joerger R, Olsson E, Granqvist CG.

Silver-based crystalline nanoparticles, microbially fabricated.

Proc Natl Acad Sci U S A. 1999 Nov 23; 96(24): 13611-13614.
PMCID: 24112

Johnson JR, Delavari P, Azar M.

Activities of a Nitrofurazone-Containing Urinary Catheter....

Antimicrob Agents Chemother. 1999 Dec; 43(12): 2990-2995.
PMCID: 89599

Chang TW, Weinstein L.

Inactivation of Treponema pallidum by Silver Sulfadiazine.

Antimicrob Agents Chemother. 1975 May; 7(5): 538-539.
PMCID: 429178

Bellantone M, Williams HD, Hench LL.

Broad-Spectrum Bactericidal Activity of Ag2O-Doped Bioactive Glass.

Antimicrob Agents Chemother. 2002 Jun; 46(6): 1940-1945.
PMCID: 127232

Galeano B, Korff E, Nicholson WL.

Inactivation of Vegetative Cells, but Not Spores, of Bacillus anthracis, B. cereus, and B.

Appl Environ Microbiol. 2003 Jul; 69(7): 4329-4331.
PMCID: 165132

Dibrov P, Dzioba J, Gosink KK, Häse CC.

Chemiosmotic Mechanism of Antimicrobial Activity of Ag+ in Vibrio cholerae.

Antimicrob Agents Chemother. 2002 Aug; 46(8): 2668-2670.
PMCID: 127333

Zhang S, Crow SA Jr.

Toxic Effects of Ag(I) and Hg(II) on Candida albicans and C. maltosa: a Flow Cytometric Evaluation.

Appl Environ Microbiol. 2001 Sep; 67(9): 4030-4035.
PMCID: 93125

Landeen LK, Yahya MT, Gerba CP.

Efficacy of copper and silver ions and reduced levels of free chlorine in inactivation of Legionella pneumophila.

Appl Environ Microbiol. 1989 Dec; 55(12): 3045-3050.
PMCID: 203221

Abad FX, Pintó RM, Diez JM, Bosch A.

Disinfection of human enteric viruses in water by copper and silver in combination with low levels of chlorine.

Appl Environ Microbiol. 1994 Jul; 60(7): 2377-2383.
PMCID: 201659

Deitch EA, Marino AA, Gillespie TE, Albright JA.

Silver-nylon: a new antimicrobial agent.

Antimicrob Agents Chemother. 1983 Mar; 23(3): 356-359.
PMCID: 184651

Mullen MD, Wolf DC, Ferris FG, Beveridge TJ, Flemming CA, Bailey GW.

Bacterial sorption of heavy metals.

Appl Environ Microbiol. 1989 Dec; 55(12): 3143-3149.
PMCID: 203237

DiRico DE Jr, Keller PB, Hartman KA.

The infrared spectrum and structure of the type I complex of silver and DNA.

Nucleic Acids Res. 1985 Jan 11; 13(1): 251-260.
PMCID: 340988

Riley TV, Mee BJ.

Susceptibility of Bacteroides spp. to heavy metals.

Antimicrob Agents Chemother. 1982 Nov; 22(5): 889-892.
PMCID: 185676

Cregan P, Yamamoto A, Lum A, VanDerHeide T, MacDonald M, Pulliam L.

Comparison of four methods for rapid detection of Pneumocystis carinii in respiratory specimens.

J Clin Microbiol. 1990 Nov; 28(11): 2432-2436.
PMCID: 268201

Haefeli C, Franklin C, Hardy K.

Plasmid-determined silver resistance in Pseudomonas stutzeri isolated from a silver mine.

J Bacteriol. 1984 Apr; 158(1): 389-392.
PMCID: 215434

Hahn RG, Hatlen JB, Kenny GE.

Comparative Poliovirus Permeability of Silver, Polycarbonate, and Cellulose Membrane Filters.

Appl Microbiol. 1970 Feb; 19(2): 317-320.
PMCID: 376675

Tobin RS, Smith DK, Lindsay JA.

Effects of activated carbon and bacteriostatic filters on microbiological quality of drinking water.

Appl Environ Microbiol. 1981 Mar; 41(3): 646-651.
PMCID: 243753

Faine S, Edelstein P, Kirby BD, Finegold SM.

Rapid presumptive bacteriological diagnosis of Legionnaires disease.

J Clin Microbiol. 1979 Jul; 10(1): 104-105.
PMCID: 273102

Tilton RC, Rosenberg B.

Reversal of the silver inhibition of microorganisms by agar.

Appl Environ Microbiol. 1978 Jun; 35(6): 1116-1120.
PMCID: 242993

Rosenkranz HS, Coward JE, Wlodkowski TJ, Carr HS.

Properties of Silver Sulfadiazine-Resistant Enterobacter cloacae.

Antimicrob Agents Chemother. 1974 Feb; 5(2): 199-201.
PMCID: 428945

Marsh PB, Simpson ME, Trucksess MW.

Effects of Trace Metals on the Production of Aflatoxins by Aspergillus parasiticus.

Appl Microbiol. 1975 Jul; 30(1): 52-57.
PMCID: 187113

Carr HS, Wlodkowski TJ, Rosenkranz HS.

Silver Sulfadiazine: In Vitro Antibacterial Activity.

Antimicrob Agents Chemother. 1973 Nov; 4(5): 585-587.
PMCID: 444599

Rosenkranz HS, Carr HS.

Silver Sulfadiazine: Effect on the Growth and Metabolism of Bacteria.

Antimicrob Agents Chemother. 1972 Nov; 2(5): 367-372.
PMCID: 444322

Berger TJ, Spadaro JA, Chapin SE, Becker RO.

Electrically Generated Silver Ions: Quantitative Effects on Bacterial and Mammalian Cells.

Antimicrob Agents Chemother. 1976 Feb; 9(2): 357-358.
PMCID: 429529

Chang TW, Weinstein L.

Prevention of Herpes Keratoconjunctivitis in Rabbits By Silver Sulfadiazine.

Antimicrob Agents Chemother. 1975 Dec; 8(6): 677-678.
PMCID: 429446

Coward JE, Carr HS, Rosenkranz HS.

Silver Sulfadiazine: Effect on the Ultrastructure of Pseudomonas aeruginosa.

Antimicrob Agents Chemother. 1973 May; 3(5): 621-624.
PMCID: 444467

Tierney JT, Sullivan R, Larkin EP, Peeler JT.

Comparison of Methods for the Recovery of Virus Inoculated into Ground Beef.

Appl Microbiol. 1973 Oct; 26(4): 497-501.
PMCID: 379834